Hypertrophic Effect of Chondrogenic Differentiation Medium Supplemented with BMP-9 and TGFß-3 in Transwell Culture

Mesenchymal stem cells are widely used in the treatment of many diseases, including osteoarthritis, due to their ability differentiate into cartilage. The high chondrogenic differentiation potential synovial fluid-derived mesenchymal increases importance these osteoarthritis treatments. Addition BMP-9 and TGF-ß3 medium, they also cause hypertrophic effects on chondrocytes. In our study, it was aimed demonstrate cell hypertrophy by adding them basal medium during vitro differentiation. passage 5 chondrocytes 1 were cultured a transwell co-culture system six experimental groups formed. Cell demonstrated examining MMP-13 RUNX-2 gene expressions, where chondrogenesis induced co-culture. Although addition increased expressions compared control, results not statistically significant. TGF-ß3, separately or combination, does significant chondrocyte hypertrophy.